TABLE 1.
Oligonucleotide primers used for RT-PCR and construction of various mutant strains
| Primer | Sequence (5′-3′)a | Use |
|---|---|---|
| CAF1 | CGTATTGCCCTAAGCTCGGTTGAA | RT-PCR |
| CAR1 | GCGATGCGCTCGATGGCTTT | RT-PCR |
| CAF2 | GCGCCCAAGCTGCTGTATGC | RT-PCR |
| CAR2 | CGATCTCGGCCAGCAAAAGGTAG | RT-PCR |
| clpF1 | GAGCTCGATCTCCGCCACATCCGTCA | Upstream flank for construction of mutant strains DC211 and DCA2422; SstI |
| clpR1 | GAATTCGAGCCGGAGACGACATAATAGAGG | Upstream flank for construction of mutant strains DC211 and DCA2422; EcoRI |
| clpF2 | GAATTCCGGCCGCGTGCTCAAGAAG | Downstream flank for construction of mutant strain DC211; EcoRI |
| clpR2 | CTCGAGGCCCAGCGTCTTGACGAACTTG | Downstream flank for construction of mutant strain DC211; SstI |
| clpF3 | GAATTCGCCATACCCGCTACTCGCTGAC | Upstream flank for construction of mutant strain DC2422; EcoRI |
| clpR3 | CTCGAGCGCGGCGATCGGACATAAG | Downstream flank for construction of mutant strain DC2422; SstI |
| PIF1 | GACGGGCGAGGGCCGCGA | Amplification of clp for construction of P1, complementation strain of mutant 5E4 |
| PIR1 | GGCGCGTCCCATCGGGGC | Amplification of clp for construction of P1, complementation strain of mutant 5E4 |
a
The underlined sequences are added restriction enzyme sites for the enzymes indicated in the column on the right.