TABLE 2.
cDNA library characterization
| Parameter | Valuea |
|---|---|
| Total | 1,230 |
| ESTs | 892 |
| Assembled sequencesb | |
| Total | 623 |
| Singletons | 76%c |
| Sequence similarityd | |
| Orphans | 3% |
| Low (<100) | 46% |
| Moderate (100-299) | 33% |
| High (>299) | 19% |
a
Given as numbers, unless otherwise indicated.
b
Assembled sequences, putatively representing different transcripts, refer to the combined sets of contigs (assembled continuous sequences) and singletons after assembly by the contig assembly program (20).
c
Fraction of singletons out of the total number of assembled sequences, an indication of the level of redundancy.
d
ESTs are distributed according to various ranges of sequence homology scores after sequence comparison with the GenBank nonredundant protein database (1).