FIG. 1.
Transposition of Tn14751 into the B. subtilis sacB gene. (A) Physical and genetic map of pMV5. pUC ori, replication origin of pUC19; pBY503 ori, replication origin of pBY503; Spr, spectinomycin resistance gene. The arrows indicate the direction of transcription. XbaI, SmaI, BamHI, and NdeI sites are indicated. (B) Lane 1, Smart Ladder (Nippon Gene-Wako, Tokyo, Japan) (the size of each band is indicated on the left); lane 2, pMV5 digested with SmaI-XbaI; lane 3, pCRA730 digested with SmaI-XbaI; lane 4, λ HindIII molecular weight marker. The arrows indicate the position of the 1.8-kb fragment in SmaI-XbaI-digested pMV5 for the band corresponding to the sacB gene (lane 2) and the position of the 20.3-kb fragment in SmaI-XbaI-digested pCRA730 for the band corresponding to the Tn14751 inserted into the sacB gene (lane 3).