TABLE 2.
Oligonucleotides used in this study
| Target | Primer | Sequence (5′-3′)a | Overhanging restriction site |
|---|---|---|---|
| IS14751L or IS14751R (fragment III)b | 1 | GGCCCTTCCGGTTTTGGGGTACAT | |
| 2 | GGCTCTTCCTGTTTTAGAGTGCAT | ||
| IS14751L, IS14751R, and pHSG398 | 3 | AGTCAGATCTAAGTGGAGCACCTAGATCGC | BglII |
| 4 | AGTCAGATCTAGTCACGCACATCTTCTGCA | BglII | |
| Kmr gene | 5 | AGTCAGATCTTGTGTCTCAAAATCTCTGA | BglII |
| 6 | AGTCAGATCTCTGAGGTCTGCCTCGTGAA | BglII | |
| Left part of Kmr gene (fragment I)b | 7 | ATGAGCCATATTCAACGGGA | |
| 8 | GGACAATTACAAACAGGAAT | ||
| Right part of Kmr gene (fragment II)b | 9 | CGTATTTCGTCTCGCTCAGG | |
| 10 | TTAGAAAAACTCATCGAGCA |
a
The restriction site overhangs used in the cloning procedure are underlined.
b
See Fig. 8.