Fig. 2.

Effects of Nampt enzyme activity inhibition by FK866 on osteoblast differentiation of CH310T1/2 cells. Cells were cultured in 6-well plate in RPMI for 2 days and then were differentiated in OBM with or without FK866 (1 nM) for 14 days. a Representative images of ALP stained differentiated osteoblast as described in “Methods”. b ALP activity analyzed by PNPP quantification as described in “Methods”. c Representative images of the differentiated osteoblast stained by Alizarin Red S as described in “Methods”. d Alizarin Red S stained differentiated osteoblast were counted using the Image J in three different view fields and the percentage positive cells over total cells were calculated. n = 3, *p < 0.05 vs FK866 untreated controls