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. 2016 Aug 29;6(1):249–260. doi: 10.5966/sctm.2016-0149

Figure 2.

Figure 2

Detection of transgene expression in chondrogenically induced human peripheral blood aspirates after rAAV‐mediated gene transfer. Aspirates were transduced with rAAV‐lacZ or rAAV‐hTGF‐β or left untreated (as described in Fig. 1), kept in chondrogenic medium for 21 days, and processed to monitor (A) lacZ expression by immunohistochemistry with corresponding histomorphometric analyses and (B, C) transforming growth factor‐β expression by immunohistochemistry with corresponding histomorphometric analyses (B) and enzyme‐linked immunosorbent assay (C) as described in Materials and Methods (original magnification, ×20; all representative data). ∗, statistically significant compared with no vector treatment; #, significant compared with rAAV‐lacZ; $, significant compared with rAAV‐hTGF‐β. Abbreviations: hTGF‐β, human transforming growth factor‐β; rAAV, recombinant adeno‐associated viral; TGF‐β, transforming growth factor‐β.