Figure 4.

Lack of teratoma formation after transplantation and stability of Muse‐AT cells. (A): Muse‐AT cells were injected (10⁶) i.t. into NODscid mice. The transplanted mice were monitored weekly for the appearance of tumors. The P19 embryonic carcinoma cell line was injected (10⁶) as the control. The mice were sacrificed when the tumors became outwardly apparent. NODscid mice injected with Muse‐AT cells did not develop teratoma during the observed period (up to 6 months). (B): H&E staining of testes showed normal tissue structure in NODscid Muse‐AT cells of the injected mice. (C): Representative normal karyotype of Muse‐AT cells that showed expression of stem cell markers. Abbreviations: d, day; i.t., intratesticular; Muse‐AT, multilineage‐differentiating stress‐enduring cells derived from adipose tissue.