Figure 1.
Adipose‐derived stem cells (ADSCs) cultured under 21% O2 accumulate oxidative DNA lesions. (A): Representative image of ADSCs immunostained with an anti‐8‐oxoG antibody. (B): Quantification of 8‐oxoG signal in nuclei of ADSCs expanded under 21% or 1% oxygen. (C): Representative image of cells subjected to alkaline comet assay. (D): Quantification of DNA in comet tail after alkaline comet assay. Error bars represent SD from three independent donors, and significance was determined by t test. Original magnification, ×63. Abbreviations: DAPI, 4′,6‐diamidino‐2‐phenylindole; 8‐oxoG, 7,8‐dihydro‐8‐oxoguanine.