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. 2016 Aug 2;6(1):68–76. doi: 10.5966/sctm.2015-0401

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© 2016 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Culture of adipose‐derived stem cells (ADSCs) under 21% oxygen shows increased DNA replication fork stalling. (A): Example of symmetric (1%) and asymmetric (21%) sister forks. IdU and CldU tracks are shown, as well as the ORI. (B): Scatter plots of the distances traveled by right‐moving and left‐moving sister forks during the IdU pulse in ADSCs. The central areas delimited with red lines contain sister forks with less than 25% length difference. The percentages of outliers (asymmetric sister forks) are indicated (lower right of plots). Abbreviations: CldU, chloro‐deoxyuridine; IdU, iodo‐deoxyuridine; ORI, origin of replication.