Figure 3.

Adipose‐derived stem cells (ADSCs) grown under high oxygen tension exhibit manifestations of replicative stress. (A): Representative image of ADSCs immunostained with an anti‐γH2AX antibody. (B): Quantification of γH2AX signal in nuclei of ADSCs expanded under 21% or 1% oxygen. (C): Representative image of ADSCs immunostained with anti‐53BP1 and anti‐cyclin‐A antibodies. (D): Quantification cyclin‐A‐negative cells containing large 53BP1 bodies. Error bars represent SD from three independent donors, and significance was determined by t test. Original magnification,×63. Abbreviations: 53BP1, p53‐binding protein 1; DAPI, 4′,6‐diamidino‐2‐phenylindole.