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. 2005 Jan;71(1):46–50. doi: 10.1128/AEM.71.1.46-50.2005

FIG. 1.

FIG. 1.

Gel electrophoresis of the 16S rRNA gene amplified with primers 530f and 1492r and with primers 27f and 1492r from samples utilized to test for DNA contamination due to cell lysis. Lane 1, DNA marker (100 bp; Promega); lanes 2 and 3, PCR products from extracellular DNA recovered from muffle furnace-treated sediment samples inoculated with V. mediterranei; lanes 4 and 5, PCR products from samples containing an internal standard of purified DNA from V. mediterranei; lanes 6 and 7, PCR products from intracellular DNA recovered from muffle furnace-treated sediment samples inoculated with V. mediterranei.