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. 2016 Aug 18;6(1):31–39. doi: 10.5966/sctm.2015-0409

Figure 5.

Figure 5

Oxidative stress analysis of PD induced pluripotent stem cell (iPSC)‐derived cardiomyocytes and PD model mice. (A): A glutathione assay was used to analyze iPSC‐derived cardiomyocytes. As indicated, Oltipraz was added at a final concentration of 10 or 50 µM (mean + SEM; n = 3). (B): A reactive oxygen species (ROS) assay was used to analyze iPSC‐derived cardiomyocytes. CellRox was added to the medium to visualize ROS production. Scale bars = 100 µm. (C): A glutathione assay was used to analyze the cardiomyocytes and skeletal muscle cells from PD mice (mean + SEM; n = 3). (D): Western blot analysis of the cardiomyocytes and skeletal muscle cells from PD mice (mean + SEM; n = 3). Abbreviations: CTRL, control; DAPI, 4′,6‐diamidino‐2‐phenylindole; GAA, acid α‐glucosidase; GSH, reduced glutathione; GSSG, oxidized glutathione; OD, optical density; PD, Pompe disease; WT, wild type.