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. 2016 Aug 8;6(1):316–329. doi: 10.5966/sctm.2016-0087

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© 2016 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Phenotypic and differential characterization study of mMSCs. Phenotypic surface antigens’ characterization of isolated mMSCs by immunocytochemistry: Sca1 (A), CD90.2 (B), CD34 (C), CD45(D), and CD11b (E). Blue fluorescent indicated Hoechst 33342 staining of nucleus, and green fluorescent indicated FITC conjugated antibodies. Scale bar: 50 µm. Osteogenic and adipogenic differentiation of mMSCs after incubation for 21 days in induction media: control MSCs (F), appearance of lipid vacuoles (G), alizarin staining for osteogenesis (H), and Oil Red O staining for adipogenesis (I). Scale bar = 100 µm. Abbreviations: DAPI, 4′,6‐diamidino‐2‐phenylindole; FITC, fluorescent isothiocyanate; mMSC, mice mesenchymal stem cell.