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. 2016 Oct 5;6(3):720–730. doi: 10.5966/sctm.2016-0104

Figure 1.

Figure 1

Cultivation at 37°C was necessary for efficient megakaryopoiesis and thrombopoiesis of human iPSC‐derived HPCs. (A): Schematic diagram of the in vitro differentiation protocol. To generate CD41a+ MKs and CD41a+ GPIX (CD42a)+ GPIbα (CD42b)+ platelets, human iPS‐sac‐derived HPCs were incubated with SCF, TPO, and heparin on C3H10T1/2 feeder cells at 24°C or 37°C during the MK differentiation phase (days 14–20) and platelet production phase (days 20–24). The numbers of MKs (B) and platelets (C) derived from iPSCs were measured under the different temperature conditions. ∗, p < .05 vs. 37°C culture condition on days 14–24 by Dunnett’s test, n ≥ 3. Abbreviations: GP, glycoprotein; HPC, hematopoietic progenitor cell; iPS, induced pluripotent stem; iPSC, induced pluripotent stem cell; MK, megakaryocyte; SCF, stem cell factor; TPO, thrombopoietin, VEGF, vascular endothelial growth factor.