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. 2016 Oct 5;6(3):720–730. doi: 10.5966/sctm.2016-0104

Figure 2.

Figure 2

CCCP‐induced GPIbα shedding was completely blocked by the ADAM17 inhibitor KP‐457 but not by p38 MAPK inhibitors. (A): Structure of KP‐457. (B): Inhibitory effects of KP‐457 and GM‐6001 on ADAM17 enzymatic activity under cell‐free conditions. n = 3. (C): Representative FACS dot plots showing the inhibitory effect of KP‐457 (15 µmol/l) and BIRB796 (10 µmol/l) on CCCP‐induced GPIbα shedding from human washed platelets. Washed platelets were incubated with 100 µmol/l CCCP in the presence of inhibitors for 24 hours at 37°C. The GPIbα+ fraction among CD41a+ platelets was measured. (D): Dose‐dependent effects of the indicated inhibitors on GPIbα retention by platelets treated with CCCP. n ≥ 3. Abbreviations: ADAM, a disintegrin and metalloproteinase; CCCP, carbonyl cyanide m‐chlorophenylhydrazone; FACS, fluorescence‐activated cell sorting; GP, glycoprotein; MAPK, mitogen‐activated protein kinase.