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. 2016 Oct 14;6(3):761–766. doi: 10.5966/sctm.2016-0175

Figure 2.

Figure 2

Human telomerase‐immortalized corneal epithelial cell (HTCEC) SP cells express ABCG2 and ABCB5. Representative images of immunohistochemistry analysis of ABCB5 and ABCG2 expression (n = 3). Expression of ABCG2 (A) and ABCB5 (B) in SP cells. Arrows indicate positive cells. (C): Image overlay of ABCG2‐ and ABCB5‐stained SP cells shows that some cells express both transporters (indicated by arrows). ABCB5 expression in SP (D) and NSP (E) cells from the same cell preparation. Green, fluorescein isothiocyanate‐conjugated secondary anti‐mouse antibody; red, rhodamine‐conjugated anti‐rabbit secondary antibody; blue, 4′,6‐diamidino‐2‐phenylindole; scale bars = 20 µm. HTCEC SP and NSP have colony‐forming ability. Phase contrast images show colony formation of SP and NSP‐sorted HTCEC on day 5 of culture (F) SP and (G) NSP. (H): Number of cells per colony plotted against cell count in SP and NSP cells in HTCEC; difference in the cell number per colony between the SP and NSP cell fractions was significant at p = .010. Note that the NSP cells form more colonies but they contain fewer cells. Scale bar = 100 µm. Abbreviations: NSP, non‐side population; SP, side population.