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. 2016 Sep 22;6(3):767–777. doi: 10.5966/sctm.2015-0374

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© 2016 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PS‐CM and PDC‐CM screening for paracrine and signaling molecules. (A): Representative profile of PS‐CM analyzed by protein array. A selection of growth factors, chemokines, and cytokines of interest is highlighted in the panel. A complete list of factors screened by the assay is presented in supplemental online Table 2. Differential analysis of conditioned media from PSs and PDCs revealed a distinctive modulation in the secretion profile of a subset of cytokines. PS/PDC ratios are plotted in (B), direct level comparison is shown in (C). Factors in plots are listed based on decreasing PS/PDC ratio. Abbreviations: O.D., optical density; PDC, pneumosphere‐derived cell; PDC‐CM, pneumosphere‐derived cell‐conditioned medium; PS, pneumosphere; PS‐CM, pneumosphere‐conditioned medium.