Figure 3.
ERCs mediate inhibition of IgM and IgG production. Pure BALB/c CD19+ B cells (5 × 105 per well) were stimulated with 2 μg/ml LPS with or without ERCs at 1:10, 1:5, 1:2, and 1:1 ratios of ERCs to B cells. After 6 days of culture, supernatants were harvested and (A) IgM or (B) IgG production was measured by enzyme‐linked immunosorbent assay. Graphs represent mean ± SE of triplicate wells. p value was determined by one‐way analysis of variance. Data shown are representative of three separate experiments. (A): ∗, p < .001 vs. the IgM level in the supernatants of B cells without ERC‐treatment. (B): ∗, p < .001 vs. the IgG level in the supernatants of B cells without ERC‐treatment. Abbreviations: ERC, endometrial regenerative cell; LPS, lipopolysaccharide.