Figure 1.

Electromechanical stimulator. (A): SolidWorks design of the printed circuit board used to perform electromechanical conditioning on six 3.5‐cm culture plates (left), and detail of a PDMS silicone substrate (right). (B): Electromechanical stimulator (left) and side view magnification (right). (C): Diagram showing the stimulation regimen applied to the cultured cells: electrical (blue) and mechanical (red) stimulations. Pulse durations and periods, delay between electrical and mechanical pulses, and mechanical pulse shape were programmable through a LabView custom application. Abbreviations: EIS, electrical impedance spectroscopy; PDMS, polydimethylsiloxane; ref., reference.