Figure 5.

The hMSC secretome as a source of neuroregulatory molecules for PD. (A): Through Bioplex‐Luminex‐based analysis, we were able to observe an active secretion of neurotrophic factors such as VEGF (highly expressed; p < .05), BDNF, IL‐6, and GDNF—important regulators/modulators on dopaminergic neuronal survival and protection. Through a combined MS analysis (B), we also found 61 proteins secreted by hMSCs that, in accordance with Gene Ontology analysis of biological processes (C) and Protein Analysis Through Evolutionary Relationships (PANTHER) pathways (D) have neuroregulatory properties such as cystatin C, glia‐derived nexin, galectin‐1, and pigment epithelium‐derived factor (PEDF) (B). Like the other neurotrophic factors, PEDF was found to be a neuroprotective molecule of the dopaminergic system. Data are presented as mean ± SEM; n = 3. Abbreviations: BDNF, brain‐derived neurotrophic factor; GDNF, glial cell line‐derived neurotrophic factor; IDA, information‐dependent acquisition; IGF, insulin‐like growth factor; IL‐6, interleukin‐6; SWATH, Sequential Window Acquisition of All Theoretical Mass Spectra; VEGF, vascular endothelial growth factor.