Figure 6.

Inducible knockout of Tgfbr2 in nestin+ cells leads to inhibit prostatic stromal hyperplasia. Nestin‐Cre-ER, Tgfbr2^flox/flox mice (Tgfbr2^−/−) and control mice (Tgfbr2^+/+) treated with saline or PE for 4 weeks. (A): Masson's trichrome staining of the prostate tissue from the mice. Panels in the bottom are at higher magnification of red boxed area in the top panels. Bar graph on the right shows the quantitative analyses of the collagen (∗). Scale bars = 50 μm in top panels, 20 μm in bottom panels. (B): Immunohistochemical staining and quantitative analyses (right) of vimentin (brown). Scale bars = 50 μm in top panels, 20 μm in bottom panels. n = 5–6 per group. Data are shown as the mean ± SD. ∗, p < .05, ∗∗, p < .01 determined by multifactorial analysis of variance. Abbreviation: PE, phenylephrine.