Figure 3.

Characteristic skin‐derived precursor (SKP) markers in bioreactor versus static culture. (A–K): Flow cytometry of single‐cell rat SKPs grown for three consecutive passages under static and bioreactor conditions was performed to assess the protein expression of characteristic SKP markers. (L): No significant difference was found in the percentage of positive cell expression between the static and bioreactor cultures. Two‐tailed t test, p = nonsignificant. Error bars represent SEM of three independent experiments. Abbreviations: APC‐A, allophycocyanin; FB, fibronectin; FSC, forward scatter; FSP1, fibroblast‐specific protein 1; PDGFR‐α, platelet‐derived growth factor receptor‐α; PE‐A, r‐phycoerythrin; α‐SMA, α‐smooth muscle actin.