Figure 5.

hiPS‐NSCs and hfNSCs display similar gene expression profiles. (A): Hierarchical clustering showing samples in columns (fibroblasts, black; hiPSCs, red; hiPS‐NSCs, light blue; and hfNSCs, blue) and genes in rows. Gene expression values are depicted according to the color scale (green, low; red, high). hiPS‐NSCs cluster separately from the parental hiPSC lines, displaying a transcriptional profile that is superimposable to that of hfNSCs. (B): Multidimensional scaling plot of distances between samples reveals clear separation of fibroblasts, hiPSC, and hNSC cell populations, with overlap of hiPS‐NSC and hfNSC samples. Each dot depicts an individual sample. Color code is as in (A) and (C). (C): Heat map showing expression of selected genes. Genes in the upper cluster are upregulated in hiPS‐NSCs and hfNSCs, whereas genes in the lower cluster are upregulated in hiPSCs. Samples are shown in columns; rows represent color‐scaled expression values (green, low; red, high). (D–G): Functional enrichment within differentially expressed genes between cell populations. For each comparison, the top significantly overrepresented functional terms are shown (Benjamini p < .5), along with their fold enrichment (horizontal bars). Abbreviations: hFib, fibroblast cell line; hfNSC, fetal‐derived human neural stem cell; hIPSC, human induced pluripotent stem cell; hiPS‐NSC, human induced pluripotent stem cell‐derived neural stem cell; ND, normal donor.