Figure 2.

An extended impedance curve (mean ± SD) of the young superlot (human adipose stem cells [hASCs] from five female donors aged 24–36 years). The hASCs were cultured in complete growth medium until induction (day 0), at which point culture medium was changed to osteogenic differentiation medium. Matrix deposition, as identified by light microscopy, was first observed during drop phase. Full light microscopy time courses can be found in supplemental online Figures 1–3. As shown through live/dead imaging, cell viability was maintained throughout the impedance drop. Scale bars = 200 μm. Abbreviation: D, day.