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. 2016 Sep 7;6(2):458–470. doi: 10.5966/sctm.2016-0177

Figure 1.

Figure 1

Increased expression of neural stem cell‐related genes in GMSCs cultured in neural medium. GMSCs were cultured in neurobasal medium supplemented with 1% N‐2 Supplement, 2% B27, 20 ng/ml EGF, and 20 ng/ml bFGF for different time periods. (A–C): Immunofluorescence studies on the expression of Nestin, SOX1, PAX6, and Vimentin. Nuclei were counterstained with DAPI (blue). Scale bars = 20 µm (×40). (D): Percentage of cells positive for each marker. ∗∗, p < .01. (E): Western blot analysis of the expression of Nestin, SOX1, PAX6, and Vimentin; graph shows densities relative to β‐actin as the internal control. Abbreviations: bFGF, basic fibroblast growth factor; DAPI, 4′,6‐diamidino‐2‐phenylindole; EGF, epidermal growth factor; GMSC, gingiva‐derived mesenchymal stem cell N‐GMSC, neural culture of gingiva‐derived mesenchymal stem cell.