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. 2016 Sep 16;6(2):656–665. doi: 10.5966/sctm.2016-0028

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© 2016 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of adipose stromal cell (ASC) graft on bone morphogenetic protein (BMP)‐2 and osteopontin (OPN) expression during periodontal healing. BMP‐2 (green; A, D, G, J, M, P) and OPN staining (magenta; B, E, H, K, N, Q), and their colocalization (C, F, I, L, O, R) 1, 6, and 12 weeks after deep periodontium grafting ±ASC. Six weeks after surgery, BMP‐2 expression extended toward the apical part of the periodontal ligament on the experimental side only. OPN expression underlined the cementum deposition and was clearly enhanced by ASC implantation in comparison with control. High magnifications of colocalization are displayed (C′, F′, I′, L′, O′, R′). Cell nuclei are in blue. Scale bar: 100 µm. Abbreviations: AB, alveolar bone; ASC, adipose stromal cell; BMP‐2, bone morphogenetic protein‐2; D, dentin; GE, gingival epithelium; OPN, osteopontin; PDL, periodontal ligament.