Table 1. Comparison of various growth parameters of the WT and PDH45 T0 and T1 transgenic plants grown in the presence of water and 200 mM NaCl, respectively.
WT water*
|
Transgenics in 200 mM NaCl
|
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Parameter | Generation | S1 | S3 | S5 | S7 | S9 | S11 | S12 | |
Transgene copy number | T0 | 1 | 1 | 1 | 1 | 3 | 1 | 1 | |
Total protein,† mg/g FW | T0 | 0.95 | 0.86 | 0.87 | 0.93 | 0.83 | 0.91 | 0.78 | 0.81 |
Chlorophyll,† mg/g FW | T0 | 8.5 | 5.5 | 6.1 | 6.3 | 4.9 | 5.7 | 5.1 | 5.9 |
Segregation ratio,‡Kr:Ks [n] | T1 | 3.5:1 [150] | 2.03:1 [225] | 2.77:1 [171] | 2.70:1 [163] | 4.2:1 [149] | 2.9:1 [183] | 3.16:1 [215] | |
Root growth inhibition,§ % | T1 | 0 | 11.0 | 21.0 | 17.0 | 17.0 | 19.0 | 16.0 | 16.0 |
Fresh weight,† g per leaf | T1 | 3.9 | 3.9 | 3.8 | 3.9 | 3.9 | 3.9 | 3.8 | 3.8 |
Height,¶ cm | T1 | 113.0 | 106 | 109 | 104 | 113 | 101 | 109 | 107 |
Flowering time,¶ days | T1 | 122.0 | 133 | 131 | 133 | 131 | 129 | 131 | 130 |
Seed weight per pod‡ | T1 | 119.0 | 118 | 119 | 118 | 120.7 | 120.4 | 122 | 123.1 |
Experiments were repeated at least three times, and the values represent means of different samples. SD in each case was <3%. In all cases, n = 15 except for T0 seeds. n is mentioned for each line in brackets.
WT plants did not survive under salinity stress
Recordings made from mature leaf. FW, fresh weight
Recordings made from seed
Recordings made from seedling
Recordings made from mature plant