Fig. 1.
Sequences upstream of the -35 element in the lacUV5 and λPR promoters, compared with the in vitro-selected full-consensus UP element (8). The -35 hexamers and consensus UP element are in shown in bold. Positions of the proximal and distal UP-element subsites are indicated. EcoRI sites used in cloning the promoters into plasmid vectors are underlined. Sequences upstream of -60 in the promoter fragments were obtained from plasmids pRLG770 or pSL6 (34–35) and are shown in Fig. 7. Numbering is with respect to the transcription start site.