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Effects of truncation of upstream DNA (to -42) on association of wild-type RNAP or αΔ235 RNAP with the lacUV5 promoter (30°C, 110 mM KCl buffer). (A) Data are displayed as a τ plot. Wild-type reconstituted RNAP and -130 to +40 lacUV5 promoter fragment (•, from Fig. 2), wild-type reconstituted RNAP and -42 to +40 lacUV5 fragment (○), and αΔ235 RNAP and -42 to +40 lacUV5 fragment (♦) are indicated. (B) Kinetic constants derived from nonlinear analysis of the data (see also Fig. 9).
