Fig. 2.

tPA promotes EW seizures in a plasminogen-independent manner. Wild-type, tPA-/-, and plasminogen-/- mice were fed an ethanol diet for 14 days. On day 15, ethanol was withdrawn, and the mice were tested for handling-induced seizures every 2 h after EW. tPA-/- mice had significantly less severe seizures (P < 0.001 at 4, 6, 8, 10, 12, and 24 h after EW, compared with wild-type mice), whereas plasminogen-/- mice displayed seizure severity similar to that of the wild-type animals. Pair-fed ethanol-naïve mice of the same genotypes did not show seizures. n = 5 for each ethanol-naïve group, and n = 6–25 for each ethanol-treated group. The results are expressed as mean ± SEM. The error bars in some data points are smaller than the symbols and therefore are not visible.