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. 2004 Dec 29;102(2):273–278. doi: 10.1073/pnas.0407239102

Table 1. Comparison of the pH dependence of the kinetic parameters of sweet potato and pig PAP.

Sweet potato PAP
Pig PAP*
pH kcat, s-1 Km, μM kcat/Km, s-1·mM-1 kcat, s-1 Km, μM kcat/Km, s-1·mM-1
3.5 810 ± 77 44 ± 6 18,000 ± 3,100 88 ± 2 62 ± 5 1,400 ± 120
4 1,700 ± 120 68 ± 5 26,000 ± 2,600 180 ± 3 200 ± 14 880 ± 60
4.5 2,100 ± 170 68 ± 10 30,000 ± 5,100 280 ± 17 390 ± 31 730 ± 73
5 2,200 ± 140 93 ± 12 23,000 ± 3,400 380 ± 12 750 ± 70 510 ± 50
6 2,400 ± 160 330 ± 14 7,400 ± 590 240 ± 3 1,700 ± 70 140 ± 6
6.5 3,200 ± 210 930 ± 33 3,500 ± 260 150 ± 4 3,900 ± 200 38 ± 2
7 3,600 ± 290 1,800 ± 120 5,000 ± 200 5 ± 2 37,000 ± 27,000 0.14 ± 0.12
8 950 ± 67 5,000 ± 450 190 ± 22 n.d. n.d. n.d.

When measuring the pH dependence of the kinetic parameters, 4-nitrophenol phosphate was used as a substrate. n.d., not determined.

*

See ref. 50