Table 1. Comparison of the pH dependence of the kinetic parameters of sweet potato and pig PAP.
Sweet potato PAP
|
Pig PAP*
|
|||||
---|---|---|---|---|---|---|
pH | kcat, s-1 | Km, μM | kcat/Km, s-1·mM-1 | kcat, s-1 | Km, μM | kcat/Km, s-1·mM-1 |
3.5 | 810 ± 77 | 44 ± 6 | 18,000 ± 3,100 | 88 ± 2 | 62 ± 5 | 1,400 ± 120 |
4 | 1,700 ± 120 | 68 ± 5 | 26,000 ± 2,600 | 180 ± 3 | 200 ± 14 | 880 ± 60 |
4.5 | 2,100 ± 170 | 68 ± 10 | 30,000 ± 5,100 | 280 ± 17 | 390 ± 31 | 730 ± 73 |
5 | 2,200 ± 140 | 93 ± 12 | 23,000 ± 3,400 | 380 ± 12 | 750 ± 70 | 510 ± 50 |
6 | 2,400 ± 160 | 330 ± 14 | 7,400 ± 590 | 240 ± 3 | 1,700 ± 70 | 140 ± 6 |
6.5 | 3,200 ± 210 | 930 ± 33 | 3,500 ± 260 | 150 ± 4 | 3,900 ± 200 | 38 ± 2 |
7 | 3,600 ± 290 | 1,800 ± 120 | 5,000 ± 200 | 5 ± 2 | 37,000 ± 27,000 | 0.14 ± 0.12 |
8 | 950 ± 67 | 5,000 ± 450 | 190 ± 22 | n.d. | n.d. | n.d. |
When measuring the pH dependence of the kinetic parameters, 4-nitrophenol phosphate was used as a substrate. n.d., not determined.
See ref. 50