Figure 3.

The proportion of CD8⁺ effector memory T-cells, expression of interferon-γ, and sTREM-1 levels were significantly increased by IL-12 stimulation but significantly decreased by rapamycin treatment. The peripheral blood mononuclear cells obtained from IPA mice, CTX + IPA mice, CTX + IPA mice treated with IL-12 or rapamycin, and control animals were detected using flow cytometry 7 days after Aspergillus fumigatus intranasal inoculation. Side scatter and CD8a were used to gate on CD8⁺ T-lymphocytes, and CD44⁺ CD45⁺ CD62^+/- cells represented CD8⁺ effector memory T-cells. The data are presented as the mean ± standard deviation. *P < 0.05. CTX: Cyclophosphamide; IPA: Invasive pulmonary aspergillosis; IL: Interleukin; sTREM-1: Soluble triggering receptors expressed on myeloid cell-1.