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. 2014 Nov 19;10(10):2975–2982. doi: 10.4161/21645515.2014.970973

Figure 2.

Figure 2.

PCR and Southern analysis of the transgenic lines. (A) PCR analysis of the transgenes correctly inserted within the targeting site of the plastid genome, Amplification of GST-L1_2xCysM gene (2582 bp) with the primers oli248 in the L1_2xCysM gene and oli252 located within the plastome, (B); Amplification of the aadA gene (1981 bp) with primers oli251 in the aadA gene and oli253 present within the plastid genome. Lanes 1-7: Seven independently generated transplastomic lines (C) Southern blot analysis of GST-L1_2xCysM transplastomic plants. Total plant DNA was digested with BglII. The DNA sequence P (773 bp) located within left insertion site (INSL) of plastid genome was amplified by PCR and served for probing in the Southern analysis. Lanes 1-7: Seven independently generated transplastomic lines analyzed for the transgene integration, M: marker, WT: wild type.