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. 2014 Oct 31;10(10):2843–2852. doi: 10.4161/21645515.2014.971641

Figure 2.

Figure 2.

The mAb166 blocking epitope of PcrV. PcrV consists of 294 amino acids. The C-terminal center region between amino acids 144–257, consisting of 114 amino acids and located between 2 coiled-coil regions, is the blocking epitope for the monoclonal anti-PcrV IgG antibody, mAb166. This blocking epitope overlaps with both the channel-forming region for erythrocyte hemolysis and the blocking region of the homologous Yersinia LcrV, based on a sequence alignment between PcrV and LcrV. mAb166 binds to this epitope and blocks the translocation of type III secretory toxins. Amino acid 204 corresponds to the hypervariable region of Yersinia LcrV.