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. 2014 Nov 1;10(10):2885–2895. doi: 10.4161/hv.29823

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Figure 5. — Neutralization titers of sera antibodies from mice immunized against EV71 and CVA16 were analyzed by pseudovirus-based assay. Sera collected from the immunized mice (n = 5 per group) at week 2, 4, 6, 8 and 10 were serially diluted (3¹ to 3⁹) after an initial 20-fold dilution and mixed with the EV71 or CVA16 pseudoviruses before measuring luciferase activity. (A) EV71 neutralization titer; (B) CVA16 neutralization titer; (C) crossneutralizati- on titer of EV71 sera at the 6th week against genogroups B (B1-B5) and C (C1-C5); (D)cross-neutralization titer of CVA16 sera at the 6th week against genogroups A and B (B1a, B1b and B2).