Table 2B.
Distribution of specific anti-CVA16 IgG subtypes in ICR immunized mice with vCA16 and iCVA16 antigens
| OD405nm of ELISA for CVA16-specific IgG |
|||||
|---|---|---|---|---|---|
| Immunogen | IgG1 OD (S.D.) | IgG2a OD (S.D.) | IgG2b OD (S.D.) | IgG3 OD (S.D.) | IgG1:IgG2a |
| vCVA16 | 0.29 (0.02) | 0.07 (0.02) | 0.36 (0.04) | 0.17 (0.01) | 4:1 |
| vCVA16+vEV71 | 0.20 (0.02) | 0.06 (0.02) | 0.3 (0.03) | 0.13 (0.02) | 3:1 |
| iCVA16 | 0.20 (0.04) | 0.05 (0.02) | 0.32 (0.03) | 0.10 (0.02) | 4:1 |
| iCVA16+iEV71 | 0.20 (0.003) | 0.05 (0.01) | 0.28 (0.02) | 0.10 (0.01) | 4:1 |
Groups of mice received vCVA16, iCVA16, vCVA16+vEV71 or iCVA16+iEV71. Mouse sera were collected by tail vein bleeding at the 6th week post-immunization and assayed for specific anti-CVA16 IgG subtypes by ELISA. Results are presented as the mean ± SD of three independent experiments.