Fig. 2.

The mRNA transcripts of AKIP1 are present in human and mouse tissues and cell lines. (A) Northern blot analysis of adult human tissues shows a transcript size of 1.35 kb found in heart and skeletal muscles. (B) In HEK 293, MCF7, and HeLa cell lines, the three splice variants were present in various amounts. (C) PCR of the human fetal brain library clones showed the presence of AKIP1a and AKIP1b. (D) In adult mouse tissues, the 1.35-kb RNA transcript of mouse AKIP was highly expressed in heart, liver, kidney, and testis and weakly expressed in other tissues. (E)(Upper) RT-PCR was similar in the adult mouse, although the levels are increased in the liver and decreased in the heart. (Lower) The levels of GAPDH in different tissues.