Skip to main content
. 2005 Jan 3;102(2):349–354. doi: 10.1073/pnas.0408608102

Fig. 6.

Fig. 6.

AKIP1 retains the C subunit in the nucleus. (A) HeLa cells were transfected with YFP-AKIP1a, and the cells were stimulated with forskolin for 0, 5, 15, and 45 min. Cells were fixed and stained for endogenous C subunit. The C subunit localized to the nucleus after forskolin treatment in cells expressing YFP-AKIP1a but not in cells expressing YFP-AKIP (residues 1–136). (B) Inhibition of nuclear translocation of endogenous C subunit by HA-tagged (residues 1–39) C subunit. HeLa cells were cotransfected with YFP-AKIP1 and HA-tagged (residues 1–39) C subunit. Cells stimulated with forskolin, fixed, and stained for endogenous C subunit. (Inset) In an independent experiment, cells were cotransfected with YFP-AKIP1 and HA-tagged (residues 1–39) C subunit and stained with the anti-HA antibody to show that the construct expressed. Nuclear translocation of the C subunit was blocked.