Figure 3.
Bacterial lysate improves antibody response against RSV A2. (A) immunoassays for IgG against RSV F (p for comparison = 0.046), (B) avidity of IgG for RSV F (p for comparison = 0.038), (C) plaque reduction neutralization test (PRNT60) for RSV A2 (p for comparison<0.01), and (D) IgG2a/IgG1 ratio against RSV F (p = 0.04) in serum from BALB/c mice obtained 60 days after intranasal infection with RSV A2 (106 PFU) in the presence or absence of lysate as described in Materials and Methods. (E) BALB/c mice were inoculated with RSV A2 (104.5 pfu) either alone or with lysate, challenged 28 days later with the same virus (106 pfu), and viral titers in the lungs of the animals were assessed 4 days after challenge. All experiments were performed in duplicates using 6-8 mice/group.