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. 2004 Dec 27;102(2):419–424. doi: 10.1073/pnas.0408197102

Fig. 2.

Fig. 2.

HA-specific CD4 Treg neither impair proliferation nor accelerate apoptosis of HA-specific CD8 T cells. Thy1.1 BALB/c mice were adoptively transferred on day -1 with different combinations of CFSE-labeled Thy1.2 HA-specific T cells and challenged with CT26 (HA-) and CT44 (HA+) tumors on day 0 as described in Fig. 1. (a) CT44 draining LN from mice that received either naïve HA-specific CD8 T cells (○) or naïve HA-specific CD8 T cells and HA-specific CD4 Treg (•) were collected at different time points, and frequency of the transferred CD8 T cells (defined as CD8+Thy1.2+) was quantified by flow cytometry. (b) CD8 T cells in CT44 draining LN were assessed on day 6 for cell division (CFSE distribution) and apoptosis (binding to annexin V). (c) Similar analysis was performed with cells retrieved in CT26 tumor draining LN at the same time point. Similar results were obtained on days 4 and 8 (data not shown). Results show mean and SD values of six or more mice from at least three independent experiments.