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. 2004 Dec 27;102(2):419–424. doi: 10.1073/pnas.0408197102

Fig. 3.

Fig. 3.

HA-specific CD4 Treg do not control the production of inflammatory cytokines by HA-specific CD8 T cells. Thy1.1 BALB/c mice were adoptively transferred on day -1 with different combinations of Thy1.2 HA-specific T cells and challenged with CT44 (HA+) tumors on day 0 as described in Fig. 1, from which CT44 draining LN cells were collected on day 6. HA-specific CD8 T cells were analyzed for IFN-γ (a) and TNF-α (b) production after 4-h restimulation with exogenously added HA512-520 and HA107-119 peptides or PMA/ionomycin. (c) HA-specific CD8 T cells were analyzed for IFN-γ production after 4-h restimulation with DCs previously pulsed with only HA512-520 or both HA512-520 and HA107-119 peptides, or PMA/ionomycin. Results show mean and SD values of nine or more mice from at least three independent experiments.