Skip to main content
. Author manuscript; available in PMC: 2017 May 24.
Published in final edited form as: J Neurochem. 2011 Aug 16;118(6):1101–1112. doi: 10.1111/j.1471-4159.2011.07391.x

Figure 4. The effects of AHA1 overexpression on CB1R-mediated MAPK activation.

Figure 4

HEK293T cells in 6-well plates were co-transfected with CB1R (0.25/μg/well) and pcDNA 3.1 or AHA1 (each at 2.25 μg/well). Subsequently, the cells were serum starved for 24 h and then stimulated with increasing concentrations of Δ9-THC for 5 min. The reactions were stopped by aspiration of the medium and addition of 200 μl lysis buffer. Ten μg of protein were separated by SDS-page and ERK 1/2 activation and total ERK 1/2 levels were detected using specific antibodies. Top panel: representative blots from four different experiments. Lower panel: Quantification of the effects of Δ9-THC on MAPK levels, open symbols indicate pcDNA 3.1 transfected cells and black symbols indicate AHA1 overexpressing cells; n=4 in each case from four different transfections, * indicates statistically significant differences compared to pcDNA 3.1 transfected cells by one-way ANOVA.