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. 2017 May 24;8:867. doi: 10.3389/fpls.2017.00867

FIGURE 5.

FIGURE 5

The early vegetative phase transition and decreased leaf emergence phenotypes in ring1a-2 ring1b-3 are dependent on SPL family genes. (A,B) The rate of leaf emergence in wild-type and ring1a-2 ring1b-3 plants grown under SD conditions. The results represent the mean ± SEM, n = 15. ∗p < 0.05. (C) The transformation of genomic RING1A or RING1B DNA into ring1a-2 ring1b-3 completely complemented the leaf emergence phenotype of the mutant. Plants of WT, ring1a-2 ring1b-3, and the complemented lines were grown under SD conditions for 4 weeks. Of 25 lines examined, 24 were rescued by the transformation of RING1A, while all 24 lines examined were rescued by the transformation of RING1B. The result represents the mean ± SEM, n > 15. ∗p < 0.05. (D) The phenotypes of ring1-2a ring1b-3, spl9-4 spl15-1, and their quadruple mutant plants. Scale bar = 1 cm. (E) The numbers of juvenile leaves, adult leaves and cauline leaves in wild-type, ring1-2a ring1b-3, and spl9-4 spl15-1 plants and the quadruple mutants. The result represents the mean ± SEM of three independent experiments. The result represents the mean ± SEM. ∗p < 0.05, n > 15. (F) The rate of leaf emergence in wild-type, ring1-2a ring1b-3, and spl9-4 spl15-1 plants and in the quadruple mutant plants grown under SD conditions. The result represents the mean ± SEM of three independent experiments. ∗p < 0.05.