Figure 4.

Morphological observation with AO/EB double staining by fluorescence microscopy (magnification, ×40). SH-SY5Y cells were seeded in 6-well plates and, following 48-h adherence, were treated with (A) 0, (B) 0.5 and (C) 0.1 mg/ml Curcuma longa extract for 48 h. Following treatment, 95 µl cell suspension was mixed with 5 µl dye mixture, containing 100 mg/l of AO and 100 mg/l of EB in phsophate-buffered saline. Subsequently, stained cells were visualized immediately under a fluorescence microscope. Viable cells were detected in the controls. Fragmented nuclei, condensed chromatin, and apoptotic cells were detected in the C. longa extract-treated cells. Representative images from three independent experiments. AO, acridine orange; EB, ethidium bromide.