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. 2017 Mar 2;13(5):1702–1710. doi: 10.3892/etm.2017.4171

Figure 5.

Figure 5.

LOX-1 is a direct target of miR-98. (A) Depiction of the miR-98 seed sequence in the wild-type 3′-UTR of LOX-1 and the mutated site. (B and C) The protein expression levels of LOX-1 were downregulated by the miR-98 mimics (60 nmol/l) and upregulated by the miR-98 inhibitor (60 nmol/l), as assessed by western blotting. (D) The mRNA expression levels of LOX-1 were downregulated by miR-98 mimics and upregulated by the miR-98 inhibitor (60 nmol/l), as assessed by reverse transcription-quantitative polymerase chain reaction. *P<0.05, **P<0.01, vs. the vehicle group. (E) Co-transfection of miR-98 with the wild-type LOX-1 3′-UTR in HEK293T cells led to a marked decrease in luciferase activity, whereas co-transfection of miR-98 with the mutant LOX-1 3′-UTR had no effect on luciferase activity. The luciferase activities were measured using the dual-luciferase reporter assay. **P<0.01, vs. the mimics control group. LOX-1, lectin-like oxidized low-density lipoprotein receptor 1; miR-98, microRNA-98; 3′-UTR, 3′-untranslated region; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; oligo, oligonucleotide.