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. 2017 Mar 20;13(5):2169–2176. doi: 10.3892/etm.2017.4239

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Protein expression of IL-1β, TNF-α and AMPK. Protein levels of IL-1β, TNF-α and AMPK were determined by western blot analysis of testicular tissue from each group 12 h after each treatment. Roscovitine was found to significantly increase the expression of AMPK compared with that in the other three groups. Furthermore, IL-1β and TNF-α expression in the case group were significantly downregulated compared with that in the LPS group. **P<0.01; ***P<0.001. Groups: Control, injected with saline; case, injected with roscovitine (5 mg/kg) dissolved in DMSO followed by injection of 5 mg/kg LPS in saline; LPS, injected with LPS only; DMSO, injected with DMSO only; LPS, lipopolysaccharide; DMSO, dimethyl sulfoxide; TNF, tumor necrosis factor; IL, interleukin; AMPK, adenosine monophosphate-activated protein kinase.