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The (A) interferon (IFN)-γ and (B) tumor necrosis factor (TNF)-α level in the splenocytes culture supernatants by sandwich ELISA assay. Harvest the supernatants from the splenocytes culture restimulated with antigen 85B (5 µg/ml) at 37°C, 5% CO2, 100% humidity for 36 h. Shown are the means ± standard deviations. PBS, phosphate-buffered saline, BCG, Bacille Calmette-Guérin; rBCG, recombinant BCG.
