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The nitric oxide (NO) level in the MDM culture supernatants detected by Griess reagent. Harvest the supernatants from the MDM culture treated with phosphate-buffered saline (PBS), Bacille Calmette-Guérin (BCG) or rBCG::Ag85B-IFN-γ at 37°C, 5% CO2, 100% humidity for 36 and 72 h. Shown are the means ± standard deviations *0.01<P<0.05. rBCG, recombinant BCG; IFN, interferon.
