Table I.

Primers used to engineer the recombinant Bacille Calmette-Guérin.

Primers	Sequence
Ag85B	Primer F: 5′-TGTGAATTCATGACAGACGTGAGCCGAAAG-3′
	(EcoRI)
	Primer R: 5′-TACGGATCCGCCGGCGCCTAACGAACTCTG-3′
	(BamH1)
IFN-γ	Primer F: 5′-TCTGGATCCATGAACGCTACACACTGC-3′
	(BamH1)
	Primer R: 5′-ACTAAGCTTTCAGCAGCGACTCCTTTTCC-3′
	(HindIII)

For construction of the pMV361-Ag85B-INF-γ fusion molecule, the coding region (with the secretory signal sequence) of Ag85B was amplified from M. tb H37Rv genome DNA using the Ag85B primers. The polymerase chain reaction (PCR) product was digested by EcoRI and BamH1. Coding sequences for IFN-γ were amplified from mouse IFN-γ cDNA using the IFN-γ primers. The PCR product was digested by BamH1 and HindIII. The two digested gene fragments were cloned into the predigested pMV361 plasmid with EcoRI and HindIII. Underlined text, restriction endonuclease sequence. M. tb, Mycobacterium tuberculosis; Ag85B, antigen 85B; INF, interferon; F, forward; R, reverse.