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. 2017 Mar 22;13(5):2515–2522. doi: 10.3892/etm.2017.4255

Figure 4.

Figure 4.

Effect of miR-148a overexpression or ERα knockdown on E2-induced MCF7 cell viability and migration. MCF7 cells transfected with miR-NC or miR-148a mimic were treated with E2 for 3 h. (A) MTT assay and (B) wound healing assay were used to determine the cell viability and migration, respectively. (C) MCF7 cells were transfected with ERα-specific siRNA, and western blot analysis was used to evaluate the protein expression of ERα. Then, MCF7 cells with or without ERα knockdown were treated with E2 for 3 h. (D) MTT assay and (E) wound healing assay were used to determine the cell viability and migration, respectively. Non-transfected MCF7 cells were used as the control. **P<0.01 vs. control. ERα, estrogen receptor α; E2, estradiol; miR, microRNA; siRNA; small interfering RNA; miR-NC, scrambled miR mimic.